Comperative ampelographic description of clones of Misket vrachanski variety
Keywords:
ampelographic characteristic; clon; DNA analysis; variety; vineAbstract
A comparatively ampelographic description of selected clones and the Misket Vrachanski vine variety was performed using the OIV methodology. From the research it was found that the studied clones and the population of the variety, in the botanical description of shoot, leaf, cluster, berry and seed, are typical representatives of the Eastern ecological-geographic group (Convarietas orientalis Negr.). Taking into account the different features characterizing the shoot tip, the young shoot, the growth force of the vines, the tendrils, the inflorescences, the mature leaf, the cluster, the berry and their mechanical properties and technological qualities, there are some significant differences in the characteristics of the separate sign. In almost all groups of indicators, there are different ampelographic features, properties or qualities in which the selected clones differ between themselves and the Misket vrachanski variety. Microsatellite analysis using the standard 9 microsatellite markers did not reveal polymorphism in the clones and Misket vrachanski variety at the DNA level. To detect differences in their genetic profiles, it is necessary to analyze a larger number of microsatellite loci used in similar studies.
References
Benin, M., Gasquez, J., Mahfoudi, A., & Bessis, R. (1988). Caracterisation biochimique des varietes. Vitis, 27(3), 157-172.
Bertsch, C., Kieffer, F., Maillot, P., Farine, S., Butterlin, G., Merdinoglu, D., & Walter, B. (2005). Genetic chimerism of Vitis vinifera cv. Chardonnay 96 is maintained through organogenesis but not somatic embryogenesis. BMC Plant Biology, 5(1), 20.
Boss, P. K., & Thomas, M. R. (2002). Association of dwarfism and floral induction with a grape ‘green revolution’mutation. Nature, 416(6883), 847-850.
Bowers, J. E., Dangl, G. S., Vignani, R., & Meredith, C. P. (1996). Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome, 39(4), 628-633.
Bowers, J., Boursiquot, J. M., This, P., Chu, K., Johansson, H., & Meredith, C. (1999). Historical genetics: the parentage of Chardonnay, Gamay, and other wine grapes of northeastern France. Science, 285(5433), 1562-1565.
Calo, A., Costacurta, A., Paludetti, G., Calo, G., Arulsekar, S., & Parfitt, D. (1989). The use of isozyme markers to characterize grape cultivars. Riv. Vitic. Enol, 1, 15-22.
Eiras-Dias, J., Sousa, S., Cabral, F., & Carralho, I. (1989). Isoenzymatic characterization of Portuguese vine varieties of V. vinifera L. Rivista Vitic. Enol., 42(1), 23-26.
Franks, T., Botta, R., Thomas, M. R., & Franks, J. (2002). Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement. Theoretical and Applied Genetics, 104(2-3), 192-199.
Maul, E., & This, P. (2008). GENRES 081-a basis for the preservation and utilization of Vitis genetic resources. In Report of a working group on Vitis, first meeting, Palić, Serbia and Montenegro, 12-14 June, 2003 (pp. 13-22). Bioversity International.
Maul, E., Topfer, R., & Eibach, R. (2008). Vitis international variety catalogue. http://www.vivc.bafz.de.
McGovern, P. (2003). Ancient Wine: the Search for the Origins of Viniculture. Princeton University Press, p. 400.
Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic acids research, 8(19), 4321-4326.
Schaefer, H. (1971). Enzympolymorphismus in rebenblättern. Phytochemistry, 10(11), 2601-2607.
Sefc, K. M., Regner, F., Turetschek, E., Glössl, J., & Steinkellner, H. (1999). Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome, 42(3), 367-373.
Sefc, K. M., Lefort, F., Grando, M. S., Scott, K. D., Steinkellner, H., & Thomas, M. R. (2001). Microsatellite markers for grapevine: a state of the art. In Molecular Biology & Biotechnology of the Grapevine (pp. 433-463). Springer, Dordrecht.
Stavrakakis, M., & Loukas, M. (1983). The between-and within-grape-cultivars genetic variation. Scientia Horticulturae, 19(3-4), 321-334.
Thomas, M. R., & Scott, N. S. (1993). Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). Theoretical and Applied Genetics, 86(8), 985-990.
Walker, A. R., Lee, E., & Robinson, S. P. (2006). Two new grape cultivars, bud sports of Cabernet Sauvignon bearing pale-coloured berries, are the result of deletion of two regulatory genes of the berry colour locus. Plant molecular biology, 62(4-5), 623-635.
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