Application of mobile fluorescence spectroscopy as a method in the determination of varietal differences in black radish (Raphanus sativus L. var. niger) after harvesting

Authors

  • Vanya Slavova Maritsa Vegetable Crops Research Institute, Plovdiv, Agricultural Academy, Sofia Bulgaria Author

DOI:

https://doi.org/10.61308/QNVB6718

Abstract

The present study aims to establish the application of fluorescence spectroscopy as a field method in the determination of varietal differences after black radish harvesting. The experimental studies were conducted on site at the farm where the black radish accessions were grown. The fluorescence analysis was carried out with a source with an emission wavelength of 285 nm and an author-developed mobile fiber-optic experimental set
up. The subjects of this research are root crops from Black Spanish round, Black Spanish long, Rattail, and Nero Tondo. The correlation between the emission signals of the samples was established. This fact allows mobile fluorescence spectroscopy to be successfully applied as a rapid tool to establish the origin of unknown black radish root crops in the presence of a rich library of spectra as an applied tool in breeding programs. The results of the experiment can be used to optimize the time for the analysis of the varietal differences of the black radish genotypes after harvest. Fluorescence spectroscopy in a fiber-optical configuration will support the process of determining the belonging of a specific variety to a given variety, even for samples of unknown origin, when it is necessary to qualify the result of accession in a short time.

References

Barimani, F., Gholami, A. A., & Nabili, M. (2020). In vitro antifungal activity of Raphanus sativus L. var. niger (black radish) and Trachyspermum ammi (ajwain) on resistant and susceptible Aspergillus fumigatus isolates. International Journal of Molecular and Clinical Microbiology, 10(2), 1360-1368.

Chen, S., Strasser, R. J., & Qiang, S. (2014). In vivo assessment of effect of phytotoxin tenuazonic acid on PSII reaction centers. Plant Physiology and Biochemistry, 84, 10-21.

Jeon, H., Oh, S., Kum, E., Seo, S., Park, Y., & Kim, G. (2022). Immunomodulatory effects of an aqueous extract of black radish on mouse macrophages via the TLR2/4-mediated signaling pathway. Pharmaceuticals, 15(11), p.1376.

Kalaji, H. M., Oukarroum, A., Alexandrov, V., Kouzmanova, M., Brestic, M., Zivcak, M., ... & Goltsev, V. (2014). Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements. Plant physiology and biochemistry, 81, 16-25.

Kalaji, H. M., Jajoo, A., Oukarroum, A., Brestic, M., Zivcak, M., Samborska, I. A., ... & Ahmad, P. (2014). The use of chlorophyll fluorescence kinetics analysis to study the performance of photosynthetic machinery in plants. In Emerging technologies and management of crop stress tolerance (pp. 347-384). Academic Press.

Kalaji, H. M., Jajoo, A., Oukarroum, A., Brestic, M., Zivcak, M., Samborska, I. A., ... & Ladle, R. J. (2016). Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions. Acta physiologiae plantarum, 38, 1-11.

Strasser, R. J., Srivastava, A., & Tsimilli-Michael, M. (2000). The fluorescence transient as a tool to characterize and screen photosynthetic samples. Probing photosynthesis: mechanisms, regulation and adaptation, 25, 445-483.

Strasser, R. J., Tsimilli-Michael, M., Srivastava, A., & Papageorgiou, G. C. (2004). Chlorophyll a fluorescence: a signature of photosynthesis. Advances in photosynthesis and respiration series (Govindjee-series editor), 19, 321-362.

Singh, B. K. (2021). Radish (Raphanus sativus L.): Breeding for higher yield, better quality and wider adaptability. Advances in Plant Breeding Strategies: Vegetable Crops: Volume 8: Bulbs, Roots and Tubers, 275-304.

Tsimilli-Michael, M., & Strasser, R. J. (2013). The energy flux theory 35 years later: formulations and applications. Photosynthesis Research, 117(1), 289-320.

Yücetepe, A., Altin, G., & Özçelik, B. (2021). A novel antioxidant source: evaluation of in vitro bioaccessibility, antioxidant activity and polyphenol profile of phenolic extract from black radish peel wastes (Raphanus sativus L. var. niger) during simulated gastrointestinal digestion. International Journal of Food Science & Technology, 56(3), 1376-1384.

Zhang, L., & Liu, J. (2016). Effects of heat stress on photosynthetic electron transport in a marine cyanobacterium Arthrospira sp. Journal of applied phycology, 28, 757-763.

Downloads

Published

25.04.2024

How to Cite

Application of mobile fluorescence spectroscopy as a method in the determination of varietal differences in black radish (Raphanus sativus L. var. niger) after harvesting. (2024). Bulgarian Journal of Crop Science, 61(2), 47-52. https://doi.org/10.61308/QNVB6718