BLOCKING THE SYSTEMIC SPREAD OF POTATO VIRUS Y IN THE TISSUES OF POTATOES BY POSTTRANSCRIPTIONAL GENE SILENCING

Authors

  • N. PETROV Institute of Soil Science, Agro Technologies and Plant Protection “N. Pushkarov”, BG – 1080 Sofi a, Bulgaria Author
  • A. TENEVA University of Forestry, BG – 1474 Sofia, Bulgaria Author
  • M. STOYANOVA Institute of Soil Science, Agro Technologies and Plant Protection “N. Pushkarov”, BG – 1080 Sofi a, Bulgaria Author
  • R. ANDONOVA University of Forestry, BG – 1474 Sofia, Bulgaria Author
  • I. DENEV University of Plovdiv, Plant Physiology and Molecular Biology Dept., BG – 4000 Plovdiv, Bulgaria Author
  • N. TOMLEKOVA Maritsa Vegetable Crops Research Institute, BG – 4000 Plovdiv, Bulgaria Author

Keywords:

potatoes, PVYn, RNAi

Abstract

Plant virus diseases are the second biggest group after the fungal diseases in agricultural production. Enormous economic loss in agriculture is caused by the virus diseases. The traditional prevention and control of the virus diseases are mainly agricultural control measures. Potato virus Y (PVY) is the type species of genus Potyvirus from family Potyviridae. It is transmitted by aphids in non-persistent manner. Using insecticides is useless against spreading the infection because the virus is transmitted in non-persistent manner. Using resistant plant cultivars is not effective because of the fast evolution of the virus mainly through mutations and recombination between its strains. In the current paper we propose a new way of controlling the virus by blocking the replication and the transmission through the plant by RNA based vaccination of plants with specific to viral HC-Pro gene dsRNAs and siRNAs. This technology will give a chance against PVY invasion without changing the valuable qualities of the sensitive to the virus cultivars of potatoes.

References

Aalto, A., P. Sarin, A. van Dijk, M. Saarma, M. Poranen, U. Arumae and D. Bamford, 2007. Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage f6 RNA-dependent RNA polymerase, RNA, 13:

–429.

Annual report on the condition and the development of agriculture, 2012.

Bawden, F. C., 1936. The viruses causing top necrosis (acronecrosis) of the potato. Annals of Applied Biology, 23: 487–497.

Bawden, F. C., 1943. Some properties of the potato viruses. Annals of Applied Biology, 30: 82–83.

Buchen-Osmond, C., 1987. Potato virus Y in Descriptions and Lists from the VIDE Database.

Chrzanowska, M., 1991. New isolates of the necrotic strain of Potato virus Y (PVYN) found recently in Poland. Potato Research, 34: 179–182.

Chrzanowska, M., 1994. Differentiation of potato virus Y (PVY) isolates. Phytopathologica Polonica, 8: 15–20.

De Bokx, J. A., 1961. Waardplanten van het aardappel-YN-virus. Tijdschrift over Plantenziekten, 67: 273–277.

Denli, A. M. and G. J. Hannon, 2003. RNAi: an ever-growing puzzle. Trends Biochem. Sci., 28: 196–201.

Dikova, B., 2005. Tobacco rattle virus (TRV) transmission by sugarbeet seeds. Biotechnology & Biotechnological Equipment, 19 (2): 87–90.

Dikova, B., 2006a. Establishment of tobacco rattle virus (TRV) in weeds and Cuscuta. Biotechnology and Biotechnological Equipment, 20 (3): 42–48.

Dikova, B., 2006b. Identifi cation of Tobacco Rattle Virus (TRV) in Sugar Beet in Bulgaria. Biotechnology and Biotechnological Equipment, 20 (3): 49–59.

Dykstra, T. P., 1936. Comparative studies of some European and American potato viruses. Phytopathology, 26: 597–606.

Hammond, S. M., E. Bernstein, D. Beach and G. J. Hannon, 2000. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature, 404: 293–296.

Hijmans, R. J and D. M. Spooner, 2001. Geographic distribution of wild potato species. American Journal of Botany (Botanical Society of America), 88: 2101–2112.

Horvath, J., 1967a. Studies on strains of potato virus Y. 3. Strain causing browning of midribs in tobacco. Acta Phytopathologica Academiae Scientiarum Hungaricae, 2: 95–108.

Horvath, J., 1967b. Studies on strains of Potato virus Y. 4. Anomalous strain. Acta Phytopathologica Academiae Scientiarum Hungaricaek, 2: 195–210.

Horvath, J., 1967c. Virulenz differenzen verschiedener Stämme und Isolate des Kartoffel-Y-virus an Capsicum-Arten und Varietäten. Acta Phytopathologica Academiae Scientiarum Hungaricae, 2: 17–37.

Hristova, D., 2002. Monitoring of lethal necrosis on tomato in Bulgaria in 2001. In: Tenth Congress of the Bulgarian Microbilogists Plovdiv, Proceedings, pp. 358–361.

Hristova, D. and P. Kaytazova, 1996. Viral diseases on tomatoes in Bulgaria during the period 1986-1994. Plant Science, 33: 57–59.

Hristova, D., P. Kaytazova and E. Kim, 1996. Viral diseases on pepper in Bulgaria during the period 1986-1994. Plant Science, 33: 72–74.

Kasschau, K. and J. Carrington, 1998. A counter defensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell, 95: 461–470.

Klinkowski, M. and K. Schmelzer, 1960. A necrotic type of potato virus Y. American Potato Journal, 37: 221–227.

Kovachevski, I., 1942. Virosis on pepper. Arch. Bulg. Agricult. Society, 25: 102–109.

Kovachevski, I., 1951. Potato virosis on tobacco. Not. of Biol. Institute, 1: 123–142.

Le Romancer, M., C. Kerlan and M. Nedellec., 1994. Biological characterization of various geographical isolates of potato virus Y inducing superfi cial necrosis on potato tubers. Plant Pathology, 43: 138–144.

Li, H. W., A. P. Lucy, H. S. Guo, W. X. Li, L. H. Ji, S. M. Wong and S. W. Ding. 1999. Strong host resistance targeted against a viral suppressor of the plant gene silencing defense mechanism.

EMBO J., 18: 2683–2691.

Mallory, A., L. Ely, T. Smith, R. Marathe, R. Anandalakshmi, M. Fagard, H. Vaucheret, G. Pruss, L. Bowman and V. Vance., 2001. HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal. Plant Cell, 13: 571–583.

Martinez, J., A. Patkaniowska, H. Urlaub, R. Luhrmann and T. Tuschl, 2002. Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Cell, 110: 563–574.

Mlotshwa, S., O. Voinnet, M. F. Mette, M. Matzke, H. Vaucheret, S. W. Ding, G. Pruss and V. B. Vance., 2002. RNA silencing and the mobile silencing signal. Plant Cell, 14: S289-S301.

Nitzany, F. E. and E. Tanne, 1962. Virus diseases of peppers in Israël. Phytopathologia Mediterranea, 4: 180–182.

Nobrega, N. R. and K. Silberschmidt, 1944. Sobre una provavel variante do virus “Y” da batatinha (Solanum virus 2, Orton) que tem a peculiaridade de provocar necroses em plantas de fumo. Arquivos do Instituto Biológico, 15: 307–330.

Nooordam, D., 1973. Identifi cation of Plant Viruses. Methods & Experiments. Centre for Agricultural Publishing and Documentation. Wageningen. Netherlands, 207 pp.

Petrov, N., 2012. Potato Virus Y on Cultivated Species of the Family Solanaceae. PhD thesis, Institute of Soil Science, Agro Technologies and Plant Protection, Bulgaria.

Petrov, N. and V. Lyubenova, 2011. Variability in P1 gene region of Potato virus Y isolates and its effect on potato crops. Symposium with reports of the Anniversary National Scientifi c Conference with Foreign Participation “Man and the Universe”, ISBN: 978-954-397-025-4, pp. 671–677.

Petrov, N., D. Hristova, C. Heinze, P. Willingman and G. Adam,

Identifi cation of the virus, causing necrotic ring spots on Smith, K. M. and R. W. Dennis, 1940. Some notes on a suspected potato tubers in Bulgaria. Plant Science, 45: 407–411. variant of Solanum virus 2 (potato virus Y). Annals of Applied Revers, F., O. Le Gall, T. Candresse and A. J. Maule, 1999. New Biology, 27: 65–70.

advances in understanding the molecular biology of plant/poty- Sturgess, O. W., 1956. Leaf shrivelling virus diseases of the tomavirus interactions. Molecular Plant-Microbe Interactions, 12: to. Queensland Journal of Agricultural Science, 13: 175–220.

–376. United Nations Food and Agricultural Organisation, 2009. In-

Salaman, R. N., 1930. Virus diseases of potato: Streak. Nature, ternational Year of the Potato 2008 – The Potato.

: 241. Vance, V. and H. Vaucheret, 2001. RNA silencing in plants – de-

Shukla, D. D., M. J. Frenkel and C. W. Ward, 1991. Structure fense and counter defense. Science, 292: 2277-2280.

and function of the potyvirus genome with special reference Van der Vlugt, R. A., J. Leunissen and R. Goldbach, 1993. Taxto the coat protein coding region. Canadian Journal of Plant onomic relationships between distinct potato virus Y isolates

Pathology, 13: 178–191. based on detailed comparisons of the viral coat proteins and Silberschmidt, K., 1956. Una doenca do tomateiro em piedade 3’ non-translated regions. Archives of Virology, 131: 361–375.

causada pelo virus Y da batatinha. Arquivos do Instituto Bi- Voinnet, O. and D. C. Baulcombe, 1997. Systemic signalling in ológico, 23: 125–150. gene silencing. Nature, 389: 553.

Silberschmidt, K., 1957. Cross-protection (“premunity”) tests with Ward, C. W. and D. D. Shukla, 1991. Taxonomy of potyviruses:

two strains of potato virus Y in tomatoes. Turrialba, 7: 34–43. current problems and possible solutions. Intervirology, 32: Silberschmidt, K. M., 1960. Types of potato virus Y necrotic to to- 269–296.

bacco: History and recent observation. American Potato Jour- Weidemann, H. L., 1988. Importance and control of potato virus nal, 37: 151–159. YN (PVYN) in seed potato production. Potato Research, 31: 85–94.

Simons, J. N., 1959. Potato virus Y appears in additional areas of peppers and tomato production in South Florida. Plant Disease Reporter, 43: 710–711.

Weidemann, H. L. and E. Maiss, 1996. Detection of the potato tu-ber necrotic ringspot strain of potato virus Y (PVYNTN) by reSmith, K. M., 1931. On the composite nature of certain potato vi- verse transcription and immunocapture polymerase chain reacrus diseases of the mosaic group. Nature, 127: 702. tion. Journal of Plant Diseases and Protection, 103: 337–345.

Downloads

Published

30.04.2015

Issue

Section

Agrochemisty and Soil Science

How to Cite

PETROV, N., TENEVA, A., STOYANOVA, M., ANDONOVA, R., DENEV, I., & TOMLEKOVA, N. (2015). BLOCKING THE SYSTEMIC SPREAD OF POTATO VIRUS Y IN THE TISSUES OF POTATOES BY POSTTRANSCRIPTIONAL GENE SILENCING. Bulgarian Journal of Agricultural Science, 21(2), 288-294. https://agriacad.eu/ojs/index.php/bjas/article/view/5307