RUMEN DEGRADABILITY OF DRY MATTER AND PROTEIN IN FOUR PROTEIN SOURCES AND THEIR RELATIONSHIPS WITH MILK PROTEIN YIELD IN DAIRY COWS
Keywords:
rumen degradability, dry matter, crude protein, relationship with milk protein yield, cowsAbstract
This study evaluated the in situ ruminal degradability of sunflower meal (SFM), soybean meal (SBM), rapeseed meal, canola type (RSM) and dry distillers grain with solubles from maize (DDGS). The same feeds are used as a sole supplementary protein sources in rations of dairy cows to estimate their effect on true milk protein yield. In situ dry matter (DM) and crude protein (CP) degradability was estimated following Orskov and McDonald (1979) method using three rumen-fi stulated cows at 0, 2, 4, 8, 16, 24 and 48 h rumen incubation time for totally 6 samples of each feed and incubation interval. Ruminal disappearance of DM of SFM and DDGS was much slower, and after 8 h of rumen incubation differences were signifi cant (P < 0.05) compared to DM disappearance of SBM and RSM. Disappearance of DM of SBM and RSM was very similar and didn’t differ significantly at any time of rumen incubation. Disappearance of CP of DDGS was slower and after 8 h of incubation differences were signifi cant (P < 0.05) compared to other three protein feeds. SFM had faster (P < 0.05) protein degradability during the fi rst 8 hours of rumen incubation compared to the other three feeds. On the opposite, SBM and RSM protein degradability was slower than that of SFM for the first 8 h of rumen incubation, but later SBM degradability was slightly better than that for SFM and RSM. There was no correlation between DM and CP values of the rapidly disappearing fraction a in different feeds. The rapidly degradable DM fraction a had higher value than protein fraction a in all tested feeds. SFM and DDGS had significantly (P < 0.05) higher easily degradable CP fraction a, compared to SBM and RSM. Rate of degradation of DM and CP of DDGS was much lower (P < 0.05) than in other feeds. Effective degradability values independently of passage rate of rumen content of DM in SFM and DDGS were significantly lower, than for SBM and RSM. Effective degradability of CP was significantly lower (P < 0.05) for DDGS than for the other three feeds. Effective degradability of SFM was the highest, followed by SBM and RSM but the differences were not signifi cant (P > 0.05). There was a relatively high correlation between protein digestible into intestine (PDI) (equal to metabolizable protein) calculated from average data for degradability published in literature (r = 0.72) or from real degradability determined in this experiment (r = 0.75). Differences between two correlation coefficients were not significant. Correlation coefficient between milk true protein yields and lysine digestible in small intestine, calculated from literature data for CP degradability into rumen was 0.57, whereas the value calculated from real rumen degradability of supplementary protein was 0.48. There was a big discrepancy between lysine supply to cows and milk protein yield especially for the diet with DDGS. Presumably microbial protein synthesis into rumen was increased when DDGS was used as source of protein in the ration. A second possibility is the better mammary extraction of amino acids which are in small shortage compared to requirements.
References
Alexandrov, A., 1997. Determination and prediction of apparent and true degradability of feeds into rumen, PhD Thesis, Research Institute for Animal Science, Kofstinbrod, Bulgaria (Bg).
Alexandrov, A., S. L. Atanassova, V. D. Peltekova, G. A. Broderick and N. A. Todorov, 1995. The use of near infrared spectroscopy for estimation of microbial contamination of in sacco forage residues and protein and dry matter degradability in the rumen. In: A. F. Nunes, A. V. Portugal, J. P. Costa and J. R. Ribeiro (Eds.) Protein Metabolism and Nutrition, Proc. 7th Int. Symp. on Protein Metabolism and Nutrition, 24–27 May 1995, vale de Santarem-Portugal, EAAP Publ. № 81, Estacao Zootecnica National Portugal, pp. 133–139.
AOAC, 2007. Offi cial Methods of Analysis of AOAC International, 18 ed., Rev. 2, Association of Offi cial Analytical Chemists International, Gaithersburg, MD, USA.
Brito, A. F. and G. A. Broderick, 2007. Effect of different protein supplements on milk production and nutrient utilization in lactating dairy cows. J. Dairy Sci., 90: 1816–1827.
Broderick, G. A., 1992. Relative value of fi sh meal versus solvent soyben meal for lactating dairy cows fed alfalfa silage as sole forage. J. Dairy Sci., 75: 174–183.
Broderck, G. A. and R. C. Cochran, 2000. In vitro amd in situ methods for estimating digestibility. In: M. K. Theodorou and J. France (Ed.) Feeding Systems and Evaluation Models, CABI Publishing, CABI International, Wallingford, England, UK, pp. 53–85.
Broderick, G. A., P. Huhtanen, S. Ahvenjarvi, S. M. Reynal and R. J. Shingfi eld, 2010. Quantifting ruminal nitrogen metabolism using the omasial sampling technique in cattle – a metaanalysis. J. Dairy Sci., 93 (7): 3216–3230.
Cherenková, M., Z. Čerešňáková and M. N. Weisbjerg, 2010. Degradation characteristics of protein in feeds for ruminants. In: G. M. Grovetto (Ed.) Energy and Protein Metabolism and Nutrition, 3rd EAAP Inter. Symp. on Energy and Protein Metabolism and Nutrition, Parma, Itali, 6-10 Sertember 2010, Wageningen Academic Publishers, pp. 725–726.
Feldet, M. A., V. L. Voss, G. A. Broderick and L. D. Satter, 1991. Chemical in vitro and in situ evaluation of heat treated soybean proteins. J. Dairy Sci., 74: 2548–2554.
Ha, J. K. and J. J. Kennelly, 1984. In situ dry matter and protein degradation of various protein sources in dairy cattle. Can. J. Anim. Sci. 64: 443-452.
Huhtanen, P. and A. N. Hristov, 2009. A meta-analysis of the effect of dietary protein concentration and degradability on milk protein yield and milk N efficiency in dairy cows. J. Dairy Sci., 92: 3222–3232.
Huhtanen, P., J. I. Nouisanen, M. Rinne, K. Kyola and H. Khalili, 2008. Utilization and partitioning of dietary nitrogen in dairy cows fed grass silage based diets. J. Dairy Sci., 91: 3589–3599.
Hvelplund, T. and J. Madsen, 1990. A study of the quantitative nitrogen metabolism in the gastrointestinal tract, and the resultant new protein evaluation system for ruminants, The AAT-PBV system. Thesis, Institute of Animal Science, The Royal Vet. and Agric. University, Copenhagen.
Kendall, E. M., J. R. Ingalls and R. J. Boila, 1991. Variability in the rumen degradability and post ruminal digestion of the dry matter, nitrogen and amino acids of canola meal. Canadian Veterinary Journal, 71 (3): 739–754.
Mjoun, K., K. F. Kalscheur, A. R. Hippen and D. J. Schingoethe, 2010b. Performance and amino acid utilization of early lactation dairy cows fed regular or reduced–fat dried distillers grains with solubles. J. Dairy Sci., 93: 3176–3191.
Mjoun, K., K. F. Kalscheur, A. R. Hippen, D. J. Schingoethe and D. E. Little, 2010a. Lactation performance and amino acid utilization of cows fed increasing amounts of reduced-fat dried distillers grains with solubles. J. Dairy Sci,.93:.288–303.
NRC, 2001. Nutrient Requirements of Dairy Cattle, 7th revised ed., National Research Council, National Academy Press, Washington, DC.
Ørskov, E. R. and I. McDonald, 1979. The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. J. Agric. Sci. (Camb.), 92: 499–503.
Pamp, B. W., K. F. Kalscheur, A. R. Hippen and D. J. Schingoethe, 2006. Evaluation of dried distillers grains versus soybean protein as a source of rumen-undegraded protein for lactating dairy cows. J. Dairy Sci., 89 (Suppl. 1): 403.
Richardson, J. M., R. G. Wilkinson and L. A. Sinclair, 2003. Synchrony of nutrient supply to the rumen and dietary energy source and their effects on the growth and metabolism of lambs. J. Anim. Sci., 81:1332–1347.
Rinne, M., J. Nousianen and P. Huhtanen, 2009. Effect of silage protein degradability and fermentation acids on metabolizable protein concentration: A meta-analysis of dairy cow production experiments. J. Dairy Sci., 92: 1633–1642.
SAS, 2003. Users Guide, version 9.1, SAS Institute Inc., Cary, NC. Schwab, C. G., 1996. Rumen-protected amino acids for dairy cattle: Progress forwards determinations lysine and methionine requirements. Anim. Feed Sci. Technol., 59: 87–101.
Todorov, N., I. Krachunov, D. Djuvinov and A. Alexandrov, 2007. Handbook of Animal Feeding, Publ. Matkom, Sofi a (Bg).
Valden, H. (ed.), 2011. NorFor – The Nordic Feed Evaluation System (EAAP Publication No. 130), Wageningen Academic Publishers, The Netherlands.
Wei, G., A. Chen, B. Zhang, P. Kong, Ch. Liu and J. Zhao, 2015. Degradability and post-ruminal digestion of dry matter, nitrogen and amino acids of three protein supplements. Asian-Australas J. Anim. Sci., 28 (4): 485–493.
Woods, V. B., A. P. Moloney and F. P. O’Mara, 2003. The nutritive value of concentrate feedstuffs for ruminant animals. Part II. In situ ruminal degradability of crude protein. Anim. Feed Sci. and Technol., 110: 131–143.
Yildiz, E. and N. Todorov, 2014. The comparison of the main protein sources for dairy cows. A review. Bulg. J. Agric. Sci., 20 (2): 428–446.
Yildiz, E., N. Todorov and K. Nedelkov, 2015. Comparison of different protein sources in ration for dairy cows. Bulg. J. Agric. Sci., 21 (1): 199–208.
Yosifov, M., 2014. Utilization of by-product of biofuel production in feeding of the sheep. PhD Thesis, Research Institute for Animal Science, Kostinbrod, Bulgaria (Bg).
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