Enzootic pneumonia of pigs diagnostic notes (review)
Keywords:
diagnosis, Enzootic pneumonia, Mycoplasma hyopneumoniae, pigsAbstract
The main purpose of this review is to analyze the methods used to diagnose the enzootic pneumonia which is one of the most significant and widespread respiratory infections in swine. This is a necessary condition so that adequate and timely measures for prevention and control of the disease can be identified. The benefits include decreasing economic losses in industrial pig farming. Discovering M. hyopneumoniae as a respiratory disease can be disappointing, as it isn’t always connected with the development of symptoms. The diagnosis of enzootic pneumonia requires a complex approach, i.e. combining clinical symptoms and lung lesions with results from various laboratory analyses. Depending of the stage which the disease has reached, and the type of probes analyzed, various clinical tests can be applied. Those tests are typically sensitive and specific and can be used for accurate diagnosis.
References
Bochev, I. (2007). Porcine respiratory disease complex (PRDC): A review. I. Etiology, epidemiology, clinical forms and pathoanatomical features. Bulgarian Journal of Veterinary Medicine, 10(3), 131-146.
Calsamiglia, M., Pijoan, C. & Bosch, G. J. (1999). Profiling Mycoplsama hyopneumoniae in farms using serology and a nested PCR technique. Journal of Swine Health and Production, 7, 263-268.
Calsamiglia, M., Pijoan C. & Trigo A. (1999). Application of a nested polymerase chain reaction assay to detect Mycoplasma hyopneumoniae from nasal swabs. Journal of Veterinary Diagnostic Investigation, 11, 246-251.
Calsamiglia, M., Collins J. E. & Pijoan C. (2000). Correlation between the presence of enzootic pneumonia lesions and detection of Mycoplasma hyopneumoniae in bronchial swabs by PCR. Veterinary Microbiology, 76(3), 299-303.
Caron, J., Ourdani, M. & Dea, S. (2000). Diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections in Pigs by PCR amplification of the p36 p46 genes. Journal of Clinical Microbiology, 38(4), 1390-1396.
Chae, C. (2016). Porcine respiratory disease complex: Interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and Mycoplasma hyopneumoniae. Veterinary Journal, 212, 1-6.
Cheikh Saad Bouh, K., Shareck F. & Dea S. (2003). Monoclonal Antibodies to Escherichia Coli-Expressed p46 and p65 membranous proteins for Specific immunodetection of Mycoplasma hyopneumoniae in Lungs of infected pigs. Clinical and Diagnostic Laboratory Immunology, 10(3), 459-468.
Christensen, G., Sorensen V. & Mousing J. (1999). Disease of the respiratory system, In: Straw B.E., D’Allaire S., Mengeling W.L., Taylor D.J. (eds.) Diseases of swine, 8th ed. Iowa State University Press, Ames., pp. 913-941.
Del Pozo Sacristán, R. (2014). Treatment and control of Mycoplasma hyopneumoniae infections. PhD Thesis, Faculty of Veterinary Medicine, Ghent University, Belgian, 189.
Djordjevic, S. P., Eamens, G. J., Romalis, L.F. & Saunders, M. M. (1994). An improved enzyme linked immunosorbent assay (ELISA) for the detection of porcine serum antibodies against Mycoplasma hyopneumoniae. Veterinary Microbiology, 39(3-4), 261-273.
Fano, E., Pijoan, C. & Dee, S. (2003). Evaluation of aerosol transmission of Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus (mixed infection) under field conditions. In: Proceedings of Leman Conference, St Paul, Minnesota, 15.
Feld, N. C., Qvist, P., Ahrens, P., Friis, N. F. & Meyling, A. (1992). A monoclonal blocking ELISA detecting serum antibodies to Mycoplasma hyopneumoniae. Veterinary Microbiology, 30, 35-46.
Georgakis, A. D., Bourtzi-Hatzopoulou, E., Kritas, S. K., Balkamos, G. C. & Kyriakis, S. C. (2002). A study on the Porcine Respiratory Disease Syndrome (PRDC): Update review and proposed measures for its control. J. Hellenic Vet. Med. Society, 53, 265-271.
Halbur, P. G. (1997). Making the diagnosis with Serology, antigen Detection and PCR Mycoplasmal Pneumonia in modern swine production units. In: Proceeding of the ISU Veterinary Medicine Seminar, p. 37.
Harasawa, R., Koshimizu, K., Takeda, O., Uemori, T., Asada, K. & Kato, I. (1991). Detection of mycoplasma DNA by the Polymerase Chain Reaction. Molecular and Cellular Probes, 5(2), 103-109.
Hipple, K., Ruebling, K., Minion, F. C., Thacker, B. & Thacker, E. (2002). A PCR assay to detect Mycoplasma hyopneumoniae in formalin fixed tissues. In: Proceedings of the 17th IPVS Congress, Ames, Iowa, 453.
Kobisch, M., Blanchard, B. & Potier Mf Le (1993). Mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to reinfection. Veterinary Research, 24(1), 67-77.
Kristensen, Ch., Vinther, J., Svensmark, B. & Baekbo, P. (2014). A field evalution of two vaccines against Mycoplasma hyopneumoniae infection in pigs. Acta Veterinaria Scandinavica, 56, 1-7.
Kurth, K. T., Hsu, T., Snook, E. R., Thacker, E. L., Thacker, B. J. & Minion, F. C. (2002). Use of a Mycoplasma hyopneumoniae nested polymerase chain reaction test to determine the optimal sampling sites in swine. Journal of Veterinary Diagnostic Investigation, 14(6), 463-469.
Leneveu, P., Robert, N., Keïta, A., Pagot, E., Pommier, P. & Tessier, P. (2005). Lung lesions in pigs at slaughter: a 2-year epidemiological study in France. Journal of Applied Research in Veterinary Medicine, 3(3), 259-265.
Leon, E. A., Madec, F., Taylor, N. M. & Kobisch, M. (2001). Seroepidemiology of Mycoplasma hyopneumoniae in pigs from farrow-to-finish farms. Veterinary Microbiology, 78(4), 331-341.
Le Potier, M. F., Abiven, P. & Kobisch, M. (1994). A blocking ELISA using a monoclonal antibody for the serological detection of Mycoplasma hyopneumoniae. Research in Veterinary Science, 56(3), 338-345.
Levonen, K. (1994). Detection of enzootic pneumonia in pig herds using an enzyme-linked immunosorbent assay in sow colostrum. Research in Veterinary Science, 56(1), 111-113.
Maes, D., Verdonck, M., Deluyker, H. & de Kruif, A. (1996). Enzootic pneumonia in pigs. Veterinary Quarterly, 18(3), 104-109.
Maes, D., Segales, J., Meyns, T., Sibila, M., Pieters, M. & Haesebrouck, F. (2008). Control of Mycoplasma hyopneumoniae infections in pigs. Veterinary Microbiology, 126(4), 297-309.
Maes, D., Pasmans, F. & Haesebrouck, F. (2011). Porcine mycoplasmas: The never ending story. In: Proc. of the 5-th Asian Pig Veterinary Society Congress, Pattaya, Thailand, 7-15.
Mattson, J. G., Bergdtröm, K., Wallgren, P. & Johansson, K. E. (1995). Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16SrRNA gene. Journal of Clinical Microbiology, 33(4), 893-897.
Minion, F. C., Kurth, K., Lovekamp, M., Ruebeling, K., Thacker, B. & Thacker, E. (2002). A novel quantitative polymerase Chain reaction test for Mycoplasma hyopneumoniae. In: Proceedings of the 17th IPVS Congress, Ames, Iowa, 77.
Mitov, I. (2000). Микоплазми. In: Mitov, G., Tsanev, N., Docheva, Yu., Avramova, R. (Eds.) Микробиология, 2nd еd. ARSO, Sofia, 441-444.
Morris, C. R., Gardner I. A, Hietala, S. K & Carpenter, T. E. (1995). Еnzootic pneumonia: Comparison of cough and Lung lesion as predictor of weight gain in Swine. Canadian Journal of Veterinary Research, 59(3), 197-204.
Opriessnig, T., Thacker, E. L., Yu, S., Fenaux, M., Meng, X. J. & Halbur, P. G. (2004). Experimental reproduction of post weaning multisystem wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Veterinary Pathology, 41, 624-640.
Pepovich, R. (2015). Разпространение, диагностика и мерки за контрол на ензоотичната пневмония в условията на промишленото свиневъдство. PhD Thesis, Faculty of Veterinary Medicine, University of Forestry, Sofia, Bulgaria, 207.
Pepovich R., Nikolov B., Hristov K., Genova, K. & Mehmedov T. (2014). Clinical and paraclinical studies in enzootic pneumonia in industrial swine-breeding of Bulgaria. Scientific Works. Series C. Veterinary Medicine, LX(1), 85-88.
Pepovich, R., Nikolov, B., Hristov, K. & Genova, K. (2017). Pathological observation in pigs naturally infected with Mycoplasma hyopneumoniae. Bulgarian Journal of Veterinary Medicine, 20(Suppl. 1), 338-344.
Pieters, M., Cline, G. S., Payne, B. J., Prado, C., Ertl, J. R & Rendahl, A. K. (2014). Intra-farm risk factors for Mycoplasma hyopneumoniae colonization at weaning age. Veterinary Microbiology, 172(3-4), 575-580.
Popova, T. (2009). Патогенни микоплазми. In: Микробиология, LTU, Sofia, 292-297.
Rautiainen, E., Tuovinen, V. & Levonen, K. (2000). Monitoring antibodies to Mycoplasma hyopneumoniae in sow colostrum - a tool to document freedom of infection. Acta Veterinaria Scandinavica, 41(3), 213-225.
Rautianen, E. & Wallgren, P. (2001). Aspects of the transmission of protection against Mycoplasma hyopneumoniae from sow to offsprings. Journal of Veterinary Medicine, 48, 55-65.
Rodríguez, F., Ramírez, G. A., Sarradell, J., Andrada, M. & Lorenzo, H. (2004). Immunhitochemical labelling of cytokines in lug lesions of pigs naturally infected with Mycoplasma hyopneumoniae. Journal of Comparative Pathology, 130(4), 306-312.
Ross, R. (1999). Mycoplasmal diseases. In: Leman, A.D., Straw, B.E., D‘Allaire, S., Mengeling, W.L., and Taylor, D.J., (еds) Diseases of swine, 8th edn. The Iowa State University Press, Ames, IA, pp. 495-509.
Ruiz, A. & Pijoan C. (2002). Use and interpretation of n-PCR from nasal swabs for Mycoplasma hyopneumoniae diagnosis. In: Proceedings of the 17th IPVS Congress, Ames, Iowa, 79.
Ruiz, A. R., Utrera, V. & Pijoan, C. (2003). Effect of Mycoplasma sow vaccination on piglet colonization at weaning. Journal of Swine Health and Production, 11(3), 131-135.
Sarradell, J., Andrada, M., Ramirez, A. S., Fernandez, A., Gomez-Villamandos, J. C., Jover, A., Lorenzo, H., Herraez, P. & Rodriguez, F. (2003). A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae. Veterinary Pathology, 40, 395-404.
Shafiev, A. P. & Kudryashov, A. A. (2002). Патологоморфологические изменения при микоплазмозной пневмонии свиней. Вет. практика, 1, 38-41.
Sibila, M. (2004). Epidemiological features of natural Mycoplasma hyopneumoniae infection in pigs in Spain. PhD Thesis, Facultat de Veterinària, Universitat autònoma de Barcelona, Spain, 121.
Sibila, M., Nofrarias, M., Lopez-Soria, S., Segales, J., Valero, O., Espinal, A. & Calsamiglia, M. (2007). Chronological study of Mycoplasma hyopneumoniae infection, seroconversion and associated lung lesions in vaccinated and non-vaccinated pigs. Veterinary Microbiology, 122, 97-107.
Sibila, M., Bernal, R., Torrents, D., Riera, P., Llopart, D., Calsamiglia, M. & Segales, J. (2008). Effect of sow vaccination against Mycoplasma hyopneumoniae on sow and piglet colonization and seroconversion, and pig lung lesions at slaughter. Veterinary Microbiology, 127(1-2), 165-170.
Sibila, M., Pieters, M., Molitor, T., Maes, D., Haesebrouck, F. & Segales, J. (2009). Current perspectives on the diagnosis and epidemiology of Mycoplasma hyopneumoniae infection. The Veterinary Journal, 181, 221-231.
Sims, L. D. & Glastonbury, J. R. (1996). Pathology of the pig, a diagnostic guide. Agriculture Victoria, 219-238.
Sitjar, M., Noyes, E. P., Simon, X. & Pijoan, C. (1996). Relationships among seroconversion to Mycoplasma hyopneumoniae, lung lesions and production parameters in pigs. Journal of Swine Health and Production, 4(6), 273-277.
Sørensen, V., Ahrens, P., Barfod, K., Feenstra, A. A., Feld, N. C., Friis, N. F., Bille-Hansen, V., Jensen, N. E. & Pedersen, M. W. (1997). Mycoplasma hyopneumoniae infection in pigs: duration of the disease and evaluation of four diagnostic assays. Veterinary Microbiology, 54, 23-34.
Stärk, K. (2000). Epidemiological investigation of the influence of environmental risk factors on respiratory diseases in swine - A literature review. The Veterinary Journal, 159, 37-56.
Strait, E. L., Erickson, B. Z. & Thacker, E. L. (2004). Analysis of Mycoplasma hyopneumoniae field isolated. In: Proceedings of AASV Congress, Des Moines, Iowa, 95-96.
Tazayan, A. (2009). Диагностика и лечебно-профилактические мероприятия при ензоотической пневмонии свиней. Диссертация, Донской Государственньiй Аграрньiй Университет, Rusia, 53-76.
Thacker, E. L. (2001). Mycoplasma diagnosis and immunity. In: Proceedings of AASV, Nashville, Tennessee, 467-469.
Thacker, E. L., Thacker, B. J. & Janke, B. H. (2001). Interaction between Mycoplasma hyopneumoniae and Swine influenza virus. Journal of Clinical Microbiology, 39(7), 2525-2530.
Thacker, E. L. (2004). Diagnosis of Mycoplasma hyopneumoniae. Animal Health Research Reviews, 5(2), 317-320.
Thacker, E. L. (2006). Mycoplasmal diseases. In: Leman, A.D., Straw, B.E., D‘Allaire, S., Mengeling, W.L., and Taylor, D.J., (еd.) Diseases of swine, 9th ed. The Iowa State University Press, Ames, IA, pp. 701-717.
Thacker, E. & Thacker, B. (2012). Mycoplasmal pneumonia of swine. Pork Information Gateway, 1-4.
Verdin, E., Kobisch, M., Bové, J.M., Garnier, M. & Saillard, C. (2000). Use of internal control in a nested-PCR assay for Mycoplasma hyopneumoniae detection and quantification in tracheobronchial washings from pigs. Molecular and Cellular Probes, 14(6), 365-372.
Verdin, E., Saillard, C., Labbé, A., Bové, J. M. & Kobisch, M. (2000). A nested PCR assay for the detection of Mycoplasma hyopneumoniae in tracheobronchial washings from pigs. Veterinary Microbiology, 76(1), 31-40.
Vicca, J. (2005). Virulence and antimicrobial susceptibility of Mycoplasma hyopneumoniae isolates from pigs. PhD Thesis, Faculty of Veterinary Medicine, Ghent University, Belgian, 219.
Vicca, J., Maes, D., Thermote, L., Peeters, J., Hasesbrouck, F. & Kruif, A. (2002). Patterns of Mycoplasma hyopneumoniae infections in Belgian farrow-to-finish pigs herds with diverging-course. Journal of Veterinary Medicine, 49, 349-353.
Villarreal, I. (2010). Epidemiology of M. hyopneumoniae infections and effect of control measures. PhD Thesis, Faculty of Veterinary Medicine, Ghent University, Belgian, 221.
Wallgren, P., Beskow, P., Fellstróm, C. & Resntröm, H. M. (1994). Porcine lung lesions at slaughter and their correlation to the incidence of infections by Mycoplasma hyopneumoniae and Actinobacillus pleuorpneumoniae during the rearing period. Journal of Veterinary Medicine, 41, 441-452.
Yeske, P. (2003). Vaccination against EP. Pig Progress, Special (respiratory diseases), 10-11.
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