Polymorphism of MC4R gene in New Zealand White and Californian rabbit breeds
Keywords:
Body weight, MC4R gene, PCR-RFLP, Polymorphism, Rabbit breedingAbstract
The aim of present work was to study the genetic variation of MC4R gene, associated with meat production and body weight in New Zealand White (NZW) and Californian (CA) rabbit breeds. Blood samples were collected from v. saphena
of 60 animals (50 does and 10 bucks) from the experimental flocks raised in rabbit farm part of Institute of Animal Science – Kostinbrod, Bulgaria. Blood samples were stored in vacuum tubes at -20°C until DNA purification. The genetic variation was determined by means of PCR-RFLP method, using specific set of primers and restriction enzyme BcuI.In both breeds were identified all three possible genotypes (GG, AG and AA) of MC4R gene, and in both studied breeds the frequency of heterozygous genotype AG was higher than the other genotypes – 0.68 for NZW and 0.53 for CA. The mutant genotype AA was with lowest frequency in both breeds – 0.12 for NZW and 0.07 for CA. This is the first study in Bulgaria of MC4R gene. Further tests are pending to establish the influence of each genotype on the growth development of several rabbit generations from both breeds.
References
Chen, M., Wang, A., Fu, J. & Li, N. (2004). Different allele frequencies of MC4R gene variants in Chinese pig Breeds. Arch. Tierz., Dummerstorf, 47(5), 463-468. https://doi.org/10.5194/aab-47-463-2004
Fontanesi, L., Scotti, E., Cisarova, K., Battista, P. D., Dall’Olio, S., Fornasini, D. & Frabetti, A. (2013). A missense mutation in the rabbit melanocortin 4 receptor (MC4R) gene is associated with finishing weight in a meat rabbit line. Animal biotechnology, 24(4), 268-277. https://doi.org/10.1080/10495398.2013.781034
Gencheva, D.G., Koynarski, Ts.V., Dafova, V. & Tanchev, S.G. (2021). Entification of nucleotide variation of growth hormone gene in rabbit populations reared in Bulgaria, World Rabbit Sci. 29, 19-29. https://doi.org/10.4995/wrs.2021.12693
Gencheva, D. G., Velikov, K. P. & Veleva, P. M. (2022). Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits. World Rabbit Science, 30(1), 95-102. https://doi.org/10.4995/wrs.2022.13127
Helal, M., Hany, N., Maged, M., Abdelaziz, M., Osama, N., Younan, Y. W., Ismail, Y., Abdelrahman, R. & Ragab, M. (2022). Candidate genes for marker-assisted selection for growth, carcass and meat quality traits in rabbits. AnimBiotechnol., 33(7), 1691-1710. DOI: 10.1080/10495398.2021.1908315. Epub 2021 Apr 19. PMID: 33872113.
Hristova, D., Tanchev, S., Velikov, K., Gonchev, P. & Georgieva, S. (2017). Rabbit growth hormone and myostatin gene polymorphisms. Open Access Journal of Agricultural Research, 2(3), 000133.DOI:10.23880/OAJAR-16000133
Hristova, D. G., Tanchev, S. G., Velikov, K. P., Gonchev, P. G. & Georgieva, S. J. (2018). Single nucleotide polymorphism of the growth hormone (GH) encoding gene in inbred and outbred domestic rabbits, World Rabbit Sci. 26(1), 49-55. https://doi.org/10.4995/wrs.2018.7211
Maharani, D., Fathoni, A., Sumadi, S., Hartatik, T. & Khusnudin, M. (2018). Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle. Journal of the Indonesian Tropical Animal Agriculture, 43(2), 87-93. https://doi.org/10.14710/jitaa.43.2.87-93
Miller, I., Rogel-Gaillard, C., Spina, D., Fontanesi, L., Almeida, A. (2014). The rabbit as an experimental and production animal: From Genomics to Proteomics. Curr. Protein Pept. Sci., 15(2), 134-145. DOI: 10.2174/1389203715666140221115135
Nahácky, J., Židek, R., Gabor, M., Miluchová, M., Bučko, O. & Kovacik, A. (2018). MC4R and PGAM2 genes polymorphism association with production traits in rabbit (Oryctolaguscuniculus). Journal of Microbiology, Biotechnology and Food Sciences, 7(5). 493-495. DOI: 10.15414/jmbfs.2018.7.5.537-539
Osaiyuwu, O. H., Bolaji, U. F. O., Adeyinka, O. A., Akinyemi, M. O. & Salako, A. E. (2020). Melanocortin 4 receptor (Mc4r) gene polymorphism and its association with body weights of some breeds of rabbit. Nigerian Journal of Animal Production, 47(2), 1-7. DOI: https://doi.org/10.51791/njap.v47i2.48
Radwan, H. A., Ateya, A. I., Abo Elfadl, E. A., Sakr, S. A., Fouda, M. M., Darwish, R. A. & El-Desoky, A. E. (2022). MC4R gene polymorphisms for classification of growth efficiency and carcass measurements in two rabbit breeds in Egypt, S. Afr. J. Anim. Sci. 52(6), 743-755. http://dx.doi.org/10.4314/sajas.v52i6.02
Song, G., Wang, Y., Wang, Y., Jiang, Y., Sun, S., Cai, H., Sun, G., Li, M., Bionaz, M. & Xu, H. (2022). Coprophagy prevention decreases the reproductive performance and Granulosa Cell Apoptosis via regulation of CTSB gene in rabbits, Front. Physiol. 13926795 DOI: 10.3389/fphys.2022.926795.
Wang, H., Liu, J., Li, J., Jia, Z. & Li, Ch. (2022). Comparative life cycle assessment of rex rabbit breeding industry chains: benefits of a circular industry chain, The International Journal of Life Cycle Assessment, 27:366–379.
Yadav, A. K., Tomar, S. S., Jha, A. K. & Singh, J. (2017). Importance of molecular markers in livestock improvement: a review. International Journal of Agriculture Innovations and Research, 5(4), 614-622.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Bulgarian Journal of Agricultural Science
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
