In vitro cultivation of bovine embryos
DOI:
https://doi.org/10.61308/YGOG1439Keywords:
bovine, SOF, KSOM, BSA, FCS, embryosAbstract
Embryo cultivation is the longest step during the in vitro embryo production process. In this study, the effect of adding 5% FCS or 3 mg/ml BSA to SOF and KSOM in vitro culture media was investigated. When adding FCS in SOF medium, a better effect on separation was observed (P < 0.05), and the percentage of blastocysts was higher - 58.6%. When BSA was added to SOF medium, the percentage of blastocysts was 40.1% - significantly lower. When cultured in KSOM medium with the addition of FCS, the division rate was lower than when cultured in SOF+FCS and the proportion of blastocysts was also slightly lower. When BSA was added to the KSOM medium, the percentage of blastocysts was almost as in the SOF+BSA group. Аt the conclusion of the conducted experiment, the addition of FCS in KSOM and SOF media is beneficial for in vitro cultivation, resulting in a higher percentage of blastocysts.
References
Aguila, L., Treulen, F., Therrien, J., Felmer, R., Valdivia, M. & Smith, L. C. (2020). Animals Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive
Approaches for New Challenges of Oocyte Competence. Animals, 10, 2196.
Baruselli, P. S., Rodrigues, C. A., Ferreira, R. M., Sales, J. N. S., Elliff, F. M., Silva, L. G., Viziack, M. P., Factor, L. & D’Occhio, M. J. (2022). Impact of oocyte donor age and breed on in vitro embryo production in cattle, and relationship of dairy and beef embryo recipients on pregnancy and the subsequent performance of offspring: A review. Reprod. Fertil. Dev., 34, 36.
Cognie, Y., Poulin, N., Locatelli, Y. & Mermillod, P. (2004). State of the art production, conservation and transfer of in-vitro-produced embryos in small ruminants. Reprod. Fertil. Dev., 16, 437–445.
Gardner, D. K. (2008). Dissection of culture media for embryos: The most important and less important components and characteristics, Reprod. Fertil. Dev., 20, 9-18.
Hasler, J. F. (2010). Synthetic media for culture, freezing and vitrification of bovine embryos. Reprod. Fertil. Dev., 22, 119-125.
Lim, K. T, Jang, G., Ko, K. H., Lee, W. W., Park, H. J., Kim, J. J, Lee, S. H., Hwang, W. S., Lee, B. C. & Kang, S. K. (2007). Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media. Theriogenology, 15, 293-302.
Nedambale, T. L., Dinnyes, A., Groen, W., Dobrinsky, J. R., Tian, X. C. & Yang, X. (2004). Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology, 62, 437-449.
Soto-Moreno, E. J., Balboula, A., Spinka, C. & Rivera, R. M. (2021) Serum supplementation during bovine embryo culture affects their development and proliferation through macroautophagy and endoplasmic reticulum stress regulation. PLoS ONE, 16.
Uddin Bhuiyan, M. M., Kang, S. K. & Lee, B. C. (2007) Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos. Zygote, 15, 189–198.
Rosenkrans, C. F., Zeng, G. Q., McNamara, G. T., Schoff, P. K. & Fist, N. L. (1993). Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod., 49, 459–462.
Xie, M., McCoski, S. R., Johnson, S. E., Rhoads, M. L. & Ealy, A. D. (2017) Combinatorial effects of epidermal growth factor, fibroblast growth factor 2 and insulin-like growth factor 1 on trophoblast cell proliferation and embryogenesis in cattle. Reprod. Fertil. Dev., 29, 419.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2024 Bulgarian Journal of Animal Husbandry
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
