Identification of MSTN/DraI polymorphism in ten Bulgarian sheep breeds
Keywords:
sheep, polymorphism, MSTN gene, PCR-RFLP methodAbstract
The success of sheep production depends largely on two important characteristics of sheep – their
growth ability and meat quality. The myostatin gene (MSTN) is one of the major regulators of skeletal
muscle growth and development and it is defined as a suitable candidate gene for genetic improvement
through marker-assisted selection. In this study by means of PCR-RFLP method was identified allelic
variants of intron 1 of MSTN gene in ten Bulgarian sheep breeds – Askanian (30 animals), Caucasian
(30 animals), Karnobat Merino (60), Local Karnobat (30), Karakachan (60), Breznik (30), CooperRed Shoumen (30), Il de France (60), Bulgarian Dairy Synthetic Population (60) and Pleven Black
headed (30). Genomic DNA was extracted from 420 blood samples. After PCR amplification, the
received 497 bp fragment of the myostatin gene was digested with the restriction enzyme DraI. The
results showed that this area of the myostatin gene is highly conservative in Bulgarian sheep breeds.
All of the investigated ten sheep breeds were monomorphic for the tested locus. Only the B allele and
genotype BB were found in all studied animals.
References
Aiello, D., Patel, K., & Lasagna, E. (2018). The myostatin gene: an overview of mechanisms of action and its relevance to livestock animals. Animal genetics, 49(6), 505-519.
Al-Barzinji, Y. M., & Ameen, A. A. (2019). Specific genes affecting body weight in Iraqi Awassi sheep. Journal of Duhok University, 22(s2), 187-195.
An, X. P., Wang, J. G., Hou, J. X., Zhao, H. B., Bai, L., Li, G., ... & Cao, B. Y. (2011). Polymorphism identification in the goat MSTN gene and association analysis with growth traits. Czech Journal of Animal Science, 56(12), 529-535.
Bellinge, R. H. S., Liberles, D. A., Iaschi, S. P. A., O’brien, P. A., & Tay, G. K. (2005). Myostatin and its implications on animal breeding: a review. Animal genetics, 36(1), 1-6.
Boman, I. A., & Våge, D. I. (2009). An insertion in the coding region of the myostatin (MSTN) gene affects carcass conformation and fatness in the Norwegian Spælsau
(Ovis aries). BMC Research Notes, 2(1), 1-5. Bozhilova-Sakova, M., Dimitrova, I., Stancheva, N., & Teneva, A. (2022). Molecular analysis of ovine calpastatin (CAST) and myostatin (MSTN) genes in lambs from three Bulgarian sheep breeds., Tradition and Modernity in Veterinary Medicine. 7 (1): 31-37.
Deb, R., Chakraborty, S., & Singh, U. (2012). Molecular markers and their application in livestock genomic research. J Vet Sci Technol, 3(2), 7579.
Dehnavi, E., Ahani Azari, M., Hasani, S., Nassiry, M. R., Mohajer, M., Khan Ahmadi, A., ... & Yousefi, S. (2012). Polymorphism of myostatin gene in intron 1 and 2 and exon 3, and their associations with yearling weight, using PCR-RFLP and PCR-SSCP techniques in
Zel sheep. Biotechnology Research International, 2012. Dimitrova, I., Bozhilova-Sakova, M., Ignatova, M.,
Genova, K., Ivanova, T., Teneva, A., Stoikova- Grigorova, R., & Koutev, V. (2019). Study of the MSTN gene in sheep of Caucasian merino and Ascanian merino breeds in Bulgaria. In Proceedings of International Congress on Domestic Animal Breeding, Genetics and Husbandry
(ICABGEH-19), Prague, Czechia, 11–13 September, 2019 (ISBN: 978-605-031-805-0) (pp. 203-206).
Dimitrova, I., Bozhilova-Sakova, M., & Iliev, M. (2017). Study of some genes associated with meat productivity in Karnobat Merino sheep breed using PCRRFLP. Journal of Agriculture and Veterinary Science (IOSR-JAVS), 10(8), 61-65.
Du, R., An, X., Chen, Y., & Qin, J. (2007). Functional analysis of the Myostatin gene promoter in sheep. Science in China Series C: Life Sciences, 50(5), 648-654.
Grade, C. V. C., Mantovani, C. S., & Alvares, L. E. (2019). Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation. Journal of animal science and biotechnology, 10(1), 1-19.
Grochowska, E., Borys, B., & Mroczkowski, S. (2019). Effects of intronic SNPs in the myostatin gene on growth and carcass traits in colored Polish merino sheep. Genes, 11(1), 2.
Jeanplong, F., Sharma, M., Somers, W. G., Bass, J. J., & Kambadur, R. (2001). Genomic organization and neonatal expression of the bovine myostatin gene. Molecular and cellular biochemistry, 220(1), 31-37.
Khalil, M. H. (2020). Molecular applications of candidate genes in genetic improvement programs in livestock. Egyptian Journal of Animal Production, 57(Suppl. Issue), 1-23.
Kolenda, M., Grochowska, E., Milewski, S., & Mroczkowski, S. (2019). The association between the polymorphism in the myostatin gene and growth traits in Kamieniec and Pomeranian sheep breeds. Small Ruminant Research, 177, 29-35.
Li, X., Liu, Z., Zhou, R., Zheng, G., Gong, Y., & Li, L. (2008). Deletion of TTTTA in 5′ UTR of goat MSTN gene and its distribution in different population groups and genetic effect on bodyweight at different ages. Frontiers of Agriculture in China, 2(1), 103-109.
Miller, S., Dykes, D., & Polesky, H. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic acids res, 16(3), 12-15.
Shafey, H. I., Mahrous, K. F., Hassanane, M. S., Mordy, M. A., & Rushdi, H. E. (2014). Genetic polymorphism of myostatin and insulin-like growth factor binding protein-3 genes in Egyptian sheep breeds. Global Veterinaria, 13(3), 419-424.
Yadav, A. K., Tomar, S. S., Jha, A. K., & Singh, J. (2017). Importance of molecular markers in livestock improvement: a review. International Journal of Agriculture Innovations and Research, 5(4), 614-622.
Zerbino, D. R., Achuthan, P., Akanni, W., Amode, M. R., Barrell, D., Bhai, J., ... & Flicek, P. (2018). Ensembl 2018. Nucleic acids research, 46(D1), D754-D761.
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2024 Bulgarian Journal of Animal Husbandry
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
