Comparison of the anesthetic effect of lemongrass and clove oil on two-year old common carp (Cyprinus carpio, Linnaeus, 1758)
Keywords:
anesthesia, lemongrass oil, clove oil, Cyprinus carpio, common carpAbstract
The aim of the present study is to examine the efficacy of lemongrass oil (Cymbopogon schoenanthus) and clove oil (Eugenia caryophyllata) by establishing the time needed for induction and recovery from anesthesia of two-year old common carp (Cyprinus carpio L.). The fish treated with lemongrass oil have an average body weight (BW, g) of 614.20 ± 162.18 g and an average total length (TL,
cm) of 36.10 ± 3.77 cm. The fish used in the experiment with clove oil have an average body weight
of 572.32 ± 191.32 g and average total length of 36.19 ± 4.0 cm. For both experiments, five treatments
are conducted with five experimental concentrations: 0.02 ml/l-1, 0.04 ml/l-1, 0.06 ml/l-1, 0.08 ml/l-1 and
0.10 ml/l-1. The method of individual treatment is applied. For each concentration, 10 fish are used to
ensure statistical significance of the results. For both essential oils the fastest anesthetic effect is registered at the highest concentration of 0.10 ml/l-1 with a significant difference between the variants (P
≤ 0.05). Based on the results, it can be concluded that the time required for anesthesia of two-year old
common carp with lemongrass oil is significantly longer compared to the time required using clove
oil. At the highest experimental concentration of lemongrass (0.10 ml/l-1) anesthesia occurs after more
than 10 min, which makes the use of this essential oil as an anesthetic ineffective in commercial or
scientific activities.
During the experiment, it has been established that clove oil is significantly more effective as anesthetic agent for two-year old common carp compared to lemongrass oil.
References
Al-Sagheer, A. A., Mahmoud, H. K., Reda, F. M., Mahgoub, S. A., & Ayyat, M. S. (2018). Supplementation of diets for Oreochromis niloticus with essential oil extracts from lemongrass (Cymbopogon citratus) and geranium (Pelargonium graveolens) and effects on growth, intestinal microbiota, antioxidant and immune activities. Aquaculture nutrition, 24(3), 1006-1014.
Ashley, P. J. (2007). Fish welfare: current issues in aquaculture. Applied Animal Behaviour Science, 104(3-4), 199-235.
Aydın, B., & Barbas, L. A. L. (2020). Sedative and anesthetic properties of essential oils and their active compounds in fish: A review. Aquaculture, 520, 734999.
Aydın, B., Akhan, S., Gümüş, E., & Özbaş, M. (2019). Anesthetic efficacy of clove oil and 2-phenoxyethanol on doctor fish, Garra rufa (Heckel, 1843). Boletim do Instituto de Pesca, 45(4).
Azambuja, C. R., Mattiazzi, J., Riffel, A. P. K., Finamor, I. A., de Oliveira Garcia, L., Heldwein, C. G., ... & Llesuy, S. F. (2011). Effect of the essential oil of Lippia alba on oxidative stress parameters in silver catfish (Rhamdia quelen) subjected to transport. Aquaculture, 319(1-2), 156-161.
Da Cunha, J. A., Heinzmann, B. M., & Baldisserotto, B. (2018). The effects of essential oils and their major compounds on fish bacterial pathogens–a review. Journal of applied microbiology, 125(2), 328-344.
Devi, V., Ushanandhini, R., Kumar, K., Maheswari, M., Sivashanmugham, A., & Jagannath, P. (2011). Antioxidant activity of Cassia auriculata Linn flowers. Pharmacology Online, 2, 490-498.
Dominic, A. D., Inasu, N. D., & Swapna, J. (2016). Efficiency of anaesthetics in combating stress in fish transportation of Etroplus maculatus (Bloch, 1975). International Journal of Current Research, 8(7), 33850-33854.
Gomulka, P., Wlasow, T., Velíšek, J., Svobodová, Z., & Chmielinska, E. (2008). Effects of Eugenol and MS222 Anaesthesia on Siberian Sturgeon Acipenser baerii Brandt. Acta Veterinaria Brno, 77(3), 447-453.
Hajek, G. J. (2011). The anaesthetic-like effect of tea tree oil in common carp Cyprinus carpio L. Aquaculture Research, 42(2), 296-300.
Hajek, G. J., Klyszejko, B., & Dziaman, R. (2006). The anaesthetic effects of clove oil on common carp, Cyprinus carpio L. Acta ichthyologica et piscatoria, 2(36), 93-97.
Hamackova, J., Kouril, J., Kozak, P., & Stupka, Z. (2006). Clove oil as an anaesthetic for different freshwater fish species. Bulgarian Journal of Agricultural Science, 12(2), 185-194.
Hoseini, S. M., Taheri Mirghaed, A., & Yousefi, M. (2019). Application of herbal anaesthetics in aquaculture. Reviews in Aquaculture, 11(3), 550-564.
Husen, M. A., & Sharma, S. (2015). Anaesthetics Efficacy of MS-222, Benzoak® vet, AQUI-S® and Clove oil on common carp (Cyprinus carpio) fry. International Journal of Research in Fisheries and Aquaculture, 5, 104-114.
Javahery, S., Nekoubin, H., & Moradlu, A. H. (2012). Effect of anaesthesia with clove oil in fish. Fish Physiology and Biochemistry, 38(6), 1545-1552.
Khumpirapang, N., Pikulkaew, S., Anuchapreeda, S., & Okonogi, S. (2018). Anesthetic activity of plant essential oils on Cyprinus carpio (koi carp). Drug Discoveries & Therapeutics, 12(1), 21-30.
Krasteva, V., Yankova, M., & Ivanova, A. (2021). Comparison of the anesthetic effect of three essential oils on carp stocking material (Cyprinus carpio L. 1758). Journal of Mountain Agriculture on the Balkans, 24(1), 42-63.
Krasteva, V., Yankova, M., & Ivanova, A. (2021). Anesthetic effect of rosemary essential oil (Rosmarinus officinalis L.) and its use for transport of Common carp (Cyprinus carpio L.) stocking material. Zhivotnovadni Nauki, 58(6), 37-46.
Kumari, P., Abidi, S., & Parwez, I. (2018). Evaluation of anaesthetizing efficacy of clove oil in Channa punctatus. International Journal of Fisheries and Aquatic Studies, 6(5), 262-266.
Mazandarani, M., Hoseini, S. M., & Dehghani Ghomshani, M. (2017). Effects of linalool on physiological responses of Cyprinus carpio (Linnaeus, 1758) and water physico‐chemical parameters during transportation. Aquaculture Research, 48(12), 5775-5781.
Mirghaed, A. T., Yasari, M., Mirzargar, S. S., & Hoseini, S. M. (2018). Rainbow trout (Oncorhynchus mykiss) anesthesia with myrcene: Efficacy and physiological responses in comparison with eugenol. Fish Physiology and Biochemistry, 44(3), 919-926.
Mitjana, O., Bonastre, C., Insua, D., Falceto, M. V., Esteban, J., Josa, A., & Espinosa, E. (2014). The efficacy and effect of repeated exposure to 2-phenoxyethanol, clove oil and tricaine methanesulphonate as anesthetic agents on juvenile Angelfish (Pterophyllum scalare). Aquaculture, 433, 491-495.
Netto, J. D. L., Oliveira, R. S., & Copatti, C. E. (2017). Efficiency of essential oils of Ocimum basilicum and Cymbopogum flexuosus in the sedation and anaesthesia of Nile tilapia juveniles. Anais da Academia Brasileira de Ciências, 89, 2971-2974.
Oladeji, O. S., Adelowo, F. E., Ayodele, D. T., & Odelade, K. A. (2019). Phytochemistry and pharmacological activities of Cymbopogon citratus: A review. Scientific African, 6, e00137.
de Oliveira, I. C., Oliveira, R. S. M., Lemos, C. H. D. P., de Oliveira, C. P. B., Felix e Silva, A., Lorenzo, V. P., ... & Copatti, C. E. (2022). Essential oils from Cymbopogon citratus and Lippia sidoides in the anesthetic induction and transport of ornamental fish Pterophyllum scalare. Fish Physiology and Biochemistry, 1-19.
Priborsky, J., & Velisek, J. (2018). A review of three commonly used fish anesthetics. Reviews in fisheries science & aquaculture, 26(4), 417-442.
Roohi, Z., & Imanpoor, M. R. (2015). The efficacy of the oils of spearmint and methyl salicylate as new anes-thetics and their effect on glucose levels in common carp (Cyprinus carpio L., 1758) juveniles. Aquaculture, 437, 327-332.
Saccol, E. M., Jerez-Cepa, I., Ourique, G. M., Pês, T. S., Gressler, L. T., Mourão, R. H., ... & Martos-Sitcha, J. A. (2018). Myrcia sylvatica essential oil mitigates molecular, biochemical and physiological alterations in Rhamdia quelen under different stress events associated to transport. Research in veterinary science, 117, 150-160.
Saccol, E. M., Toni, C., Pês, T. S., Ourique, G. M., Gressler, L. T., Silva, L. V., ... & Pavanato, M. A. (2017). Anaesthetic and antioxidant effects of Myrcia sylvatica (G. Mey.) DC. and Curcuma longa L. essential oils on tambaqui (Colossoma macropomum). Aquaculture Research, 48(5), 2012-2031.
Sampaio, F. D., & Freire, C. A. (2016). An overview of stress physiology of fish transport: changes in water quality as a function of transport duration. Fish and Fisheries, 17(4), 1055-1072.
Sánchez-Muros, M. J., Sánchez, B., Barroso, F. G., Toniolo, M., Trenzado, C. E., & Rus, A. S. (2017). Effects of rearing conditions on behavioural responses, social kinetics and physiological parameters in gilthead sea bream Sparus aurata. Applied Animal Behaviour Science, 197, 120-128.
Dos Santos, A. C., Junior, G. B., Zago, D. C., Zeppenfeld, C. C., da Silva, D. T., Heinzmann, B. M., ... & da Cunha, M. A. (2017). Anesthesia and anesthetic action mechanism of essential oils of Aloysia triphylla and Cymbopogon flexuosus in silver catfish (Rhamdia quelen). Veterinary anaesthesia and analgesia, 44(1), 106-113.
Sladky, K. K., Swanson, C. R., Stoskopf, M. K., Loomis, M. R., & Lewbart, G. A. (2001). Comparative efficacy of tricaine methanesulfonate and clove oil for use as anesthetics in red pacu (Piaractus brachypomus). American journal of veterinary research, 62(3), 337-342.
Sneddon, L., Wolfenden, D., & Thomson, J. (2016). Stress management and welfare. In: Fish Physiology: Biology of Stress in Fish. Cambridge, MA: Academic Press, 463-539.
Souza, C., Baldissera, M., Salbego, J., Lopes, J., Vaucher, R., Mourão, R., Caron, B., Heinzmann, B., Silva, L., & Baldisserotto, B. (2017). Physiological responses of silver catfish to anesthesia withessential oils from two different chemotypes of Lippia alba. Neotropical Ichthyology, 15, e160083.
Souza, C. D. F., Baldissera, M. D., Baldisserotto, B., Heinzmann, B. M., Martos-Sitcha, J. A., & Mancera, J. M. (2019). Essential oils as stress-reducing agents for fish aquaculture: a review. Frontiers in physiology, 10, 785.
de Freitas Souza, C., Baldissera, M. D., Bianchini, A. E., da Silva, E. G., Mourão, R. H. V., da Silva, L. V. F., ... & Baldisserotto, B. (2018). Citral and linalool chemotypes of Lippia alba essential oil as anesthetics for fish: a detailed physiological analysis of side effects during anesthetic recovery in silver catfish (Rhamdia quelen).Fish Physiology and Biochemistry, 44(1), 21-34.
Sutili, F. J., Gatlin III, D. M., Heinzmann, B. M., & Baldisserotto, B. (2018). Plant essential oils as fish diet additives: benefits on fish health and stability in feed. Reviews in Aquaculture, 10(3), 716-726.
Taheri Mirghaed, A., Ghelichpour, M., Zargari, A., & Yousefi, M. (2018). Anaesthetic efficacy and biochemical effects of 1, 8-cineole in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792). Aquaculture Research, 49(6), 2156-2165.
Velíšek, J., & Svobodova, Z. (2004). Anaesthesia of common carp (Cyprinus carpio L.) with 2-phenoxyethanol: acute toxicity and effects on biochemical blood profile. Acta Veterinaria Brno, 73(2), 247-252.
Wagner, G. N., Singer, T. D., & Scott McKinley, R. (2003). The ability of clove oil and MS-222 to minimize handling stress in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture research, 34(13), 1139-1146.
Zeppenfeld, C. C., Toni, C., Becker, A. G., dos Santos Miron, D., Parodi, T. V., Heinzmann, B. M., ... & Baldisserotto, B. (2014). Physiological and biochemical responses of silver catfish, Rhamdia quelen, after transport in water with essential oil of Aloysia triphylla (L’Herit) Britton. Aquaculture, 418, 101-107.
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